Elisa JM Raineri

Development of tracers and models for imagining of S. aureus infections and efficacy of novel antibiotics

New antimicrobials need to be subjected to extensive antimicrobial testing, which includes efficacy tests in appropriate small animal models. ESR 4.2 will develop specific in vivo systems where the activity of newly developed antimicrobials can be monitored non-invasively and in real time with the help of near-infrared fluorescent tracers that are either based on existing antibiotics or bacteria-specific monoclonal antibodies. Proof-of-principle for this concept was obtained in studies where implant, soft tissue or skin infections by Staphylococcus aureus, a major human pathogen, were monitored with fluorescently labeled vancomycin or a specific monoclonal antibody (van Oosten et al., Nat. Commun. 2013; 4:2584). By analogy specific tracers that target different groups of pathogens will be developed and tested. The impact of such tracers will be high, as they may ultimately be applicable in the early-stage non-invasive detection of infections in patients.

Department: Medical Microbiology, UMCG, GSMS
Principal investigator(s): Jan Maarten van Dijl

Elisa JM Raineri


Raineri, E. J. M., Altulea, D., & van Dijl, J. M. (2021). Staphylococcal trafficking and infection—from ‘nose to gut’ and back. FEMS Microbiology Reviews. https://doi.org/10.1093/femsre/fuab041  

Raineri, E. J. M., Yedavally, H., Salvati, A., & van Dijl, J. M. (2020). Time-resolved analysis of Staphylococcus aureus invading the endothelial barrier. Virulence, 11(1), 1623-1639. https://doi.org/10.1080/21505594.2020.1844418  

Bispo, M., Anaya-Sanchez, A., Suhani, S., Raineri, E. J. M., López-Álvarez, M., Heuker, M., Szymański, W., Romero Pastrana, F., Buist, G., Horswill, A. R., Francis, K. P., van Dam, G. M., van Oosten, M., & van Dijl, J. M. (2020). Fighting Staphylococcus aureus infections with light and photoimmunoconjugates. JCI Insight, 5(22). https://doi.org/10.1172/jci.insight.139512 

Zoller, S. D., Park, H. Y., Olafsen, T., Zamilpa, C., Burke, Z. D. C., Blumstein, G., Sheppard, W. L., Hamad, C. D., Hori, K. R., Tseng, J.-C., Czupryna, J., McMannus, C., Lee, J. T., Bispo, M., Romero Pastrana, F., Raineri, E. J. M., Miller, J. F., Miller, L. S., van Dijl, J. M., Francis, K. P., & Bernthal, N. M. (2019). Multimodal imaging guides surgical management in a preclinical spinal implant infection model. JCI Insight, 4(3). https://doi.org/10.1172/jci.insight.124813  

Palma Medina, L. M., Becker, A.-K., Michalik, S., Yedavally, H., Raineri, E. J. M., Hildebrandt, P., Gesell Salazar, M., Surmann, K., Pförtner, H., Mekonnen, S. A., Salvati, A., Kaderali, L., van Dijl, J. M., & Völker, U. (2019). Metabolic Cross-talk Between Human Bronchial Epithelial Cells and Internalized Staphylococcus aureus as a Driver for Infection*. Molecular & Cellular Proteomics, 18(5), 892-908. https://doi.org/10.1074/mcp.RA118.001138